This study proposes a new, efficient, and selective method for the analysis of cocaine in oral fluid. For this purpose, an extractive phase with restricted access materials (RAM) capable of extract and pre-concentrate low molecular weight analytes and simultaneously exclude macromolecules was developed for disposable pipette extraction (DPX) followed by liquid chromatography coupled to mass spectrometry (LC-MS) analyses. The proposed method was optimized using a 24 factorial design which pointed higher extraction efficiency under the following conditions: pH 9, 10 cycles of extraction (aspirate/dispense), 3 cycles of desorption (aspirate/dispense) and acetonitrile as desorption solvent. The DPX-RAM/LC-MS method showed linearity range from 10 to 100 ng mL-1 with determination coefficient (R2) = 0.999, accuracy values ranged from -0.9 to 4.3%, precision values ranged from 1.7 to 9.1% and recovery values ranged from 97.9 to 101.1%. The benefits of this method are that it can be performed in few minutes and that the analytical performances, including the limit of detection (3.13 ng mL-1), limit of quantitation (10 ng mL-1) and linear range were found to be in agreement with other methods used for similar analysis.

Due to the high sucrose content, RB002504 is indicated for harvesting in the early harvest period, from September to November, in the Northeastern region of Brazil. Planting on medium texture soils is recommended. The cultivar is highly responsive to ideal production environments and resistant to the major sugarcane diseases.

In the Northeast of Brazil, sugarcane cultivar RB962962 is harvested at the end of the cycle, between December and February, with a high sugar yield per area. Recommended for sandy soils of medium texture and fertility, it is resistant to the major diseases and fast-growing in plant and ratoon crops.

OBJETIVO: Avaliar a importância da ecocardiografia sob estresse pela dobutamina (EED) na estratificação de risco de pacientes com angina instável (AI) de baixo a moderado risco, quanto à capacidade de predizer os eventos clínicos combinados (morte de causa cardiovascular, infarto agudo do miocárdio (IAM), AI recorrente, necessidade de revascularização miocárdica) no seguimento de 6 meses. MÉTODOS: Estudo prospectivo, multicêntrico. Os pacientes incluídos estavam internados, assintomáticos havia 24 horas e a medicação em uso não era suspensa para a realização do exame. O EED era realizado preferencialmente até 72 horas da chegada ao hospital. RESULTADOS: Foram avaliados 95 pacientes consecutivos. O EED foi positivo para isquemia em 40 pacientes (42,1%) e em 55 (57,9%), foi negativo. Eventos ocorreram em 28 pacientes, 26 dos quais tinham o EED positivo para isquemia miocárdica. Os outros 67 pacientes não tiveram eventos; desses, 53 tinham o EED negativo. A sensibilidade, especificidade, acurácia, valor preditivo positivo e negativo do teste frente aos desfechos foram: 92,9%, 79,1%, 83,2%, 65% e 96,4%, respectivamente. Sobrevida livre de eventos após 6 meses para os pacientes com EED negativo foi de 96%, comparada com 35% nos que tiveram o EED positivo (p<0,001). A análise univariada identificou classificação da AI, fração de ejeção do VE, índice de movimentação parietal do VE pré e pico, resultado do EED e antecedentes de IAM prévio como fatores prognósticos associados com os desfechos. Somente a variável resultado do EED permaneceu com uma associação significativa com o desfecho através da análise multivariada (p<0,01). CONCLUSÃO: O EED apresentou excelente valor preditivo negativo, permitindo alta hospitalar precoce dos pacientes, sem necessidade de exames adicionais. O resultado do exame foi o único fator prognóstico independente para os eventos.

OBJECTIVE: To evaluate the role of dobutamine stress echocardiography (DSE) in the risk stratification of low to moderate risk unstable angina (UA) patients, to predict the combined clinical outcome of cardiovascular death, myocardial infarction (MI), recurrent UA and the need of revascularization procedures in a 6 month period. METHODS: Multicenter prospective study. Patients should be admitted to the hospital and asymptomatic in the last 24 hours. The exam was performed up to 72 hours from the hospital admission and no medication was stopped prior to the test. RESULTS: Ninety-five consecutive patients were evaluated by DSE. Forty patients (42,1%) had a positive ischemic test and fifty five (57,9%) had a negative one. Clinical events occurred in twenty eight patients, twenty six of whom had a positive test. The rest of the patients (67) did not have clinical events and fifty three of them, had a negative test. The sensibility, specificity, accuracy, positive predictive value and negative predictive value of the test related to the clinical events were: 92,9%, 79,1%, 83,2%, 65% and 96,4%, respectively. Event-free survival after 6 months for pacients with a negative DSE was 96% compared to 35% for those with a positive DSE (p<0,001). The UA classification, left ventricular ejection fraction, rest and peak wall motion score index, DSE result and history of previous MI were associated with the combined end point by univariate analysis. The test result was the only independent predictor of cardiac events by multivariate analysis (p<0.001). CONCLUSION: O DSE has shown an excellent negative predictive value allowing for early hospital discharge without further exams. The positive test result was the only independent predictor for adverse cardiac events.

As más oclusões de Classe III são as de menor incidência em nossas clínicas. Porém, a intervenção precoce têm-se mostrado muito importante para que não haja um agravamento do quadro, influenciando o comportamento psicossocial do paciente. Neste artigo serão demonstrados os resultados com um aparelho muito simples de usar, com boa colaboração dos pacientes e resultados animadores.

Early treatment in patients with Class III maloclusion can avoid psychological disordes.The effects of orthopedic therapy an mandibular growth with Batista’s reverse arch is showd in this case, indicating that is possible to solve this maloclusion with efficacy.